Set background color to white using the Color Picker Tool↑ or the Color Picker window↑.Analyze Line Graph will assume that the graph is displayed on a white background so images with darker backgrounds must be adjusted beforehand ( see e.g., Edit ▷ Invert ↑ and Process ▷Binary ▷ Make Binary↑). Make sure your graph is a grayscale image ( Image ▷ Type ▷↑8-bit). For practice, use the File ▷ Open Samples ▷↑Line Graph (21K) sample image. The following procedure describes how to use it: This command uses the Particle Analyzer↑ to extract sets of coordinate data from digitized line graphs. Select Gels ▷↑Label Peaks to label each measured peak with its size as a percent of the total size of the measured peaks.If necessary, scroll the image vertically by holding down the space bar and dragging. For each peak, measure the size by clicking inside the peak with the Wand Tool↑.To access to all the lanes, it may be necessary to scroll the image vertically using the Scrolling Tool↑ (Hold down the space bar to temporarily switch to this tool). Note that you can hold Shift to constrain lines to be either horizontal or vertical. Use the Straight Line Selection Tool↑ to draw base lines and/or drop lines so that each peak of interest defines a closed area (ImageJ will automatically switch to the Straight Line tool).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |